beta-2 Microglobulin Protein (AA 21-119) (hFc Tag)

Catalog No. ABIN7273889

Recombinant beta-2 Microglobulin protein expressed in HEK-293 Cells.

Quick Overview for beta-2 Microglobulin Protein (AA 21-119) (hFc Tag) (ABIN7273889)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-119

Purification tag / Conjugate: This beta-2 Microglobulin protein is labelled with hFc Tag.

Purpose: Human B2M/beta 2-Microglobulin Protein

Sequence: Ile21-Met119

Characteristics: Recombinant Human B2M/beta 2-Microglobulin Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ile21-Met119.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human B2M on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human B2M is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: B2M

Background: To assess whether beta-2 microglobulin (B2M) has effects on articular chondrocytes that would implicate B2M involvement in osteoarthritis (OA) pathogenesis.The average B2M level in OA synovial fluid is significantly higher than that found in normal synovial fluid. B2M is highly expressed in OA cartilage and synovial fluid compared to normal, and suggest that B2M may have effects on chondrocyte function that could contribute to OA pathogenesis.

Molecular Weight: 38.4 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process