beta-2 Microglobulin Protein (AA 21-119) (hFc Tag)

Catalog No. ABIN7273890

This Recombinant beta-2 Microglobulin protein is produced in HEK-293 Cells.

Quick Overview for beta-2 Microglobulin Protein (AA 21-119) (hFc Tag) (ABIN7273890)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-119

Purification tag / Conjugate: This beta-2 Microglobulin protein is labelled with hFc Tag.

Purpose: Mouse B2M/beta 2-Microglobulin Protein

Sequence: Ile21-Met119

Characteristics: Recombinant Mouse B2M/beta 2-Microglobulin Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ile21-Met119.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse B2M on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse B2M is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: B2M

Background: The genetic and functional analysis of β2-microglobulin (B2M), a component of the HLA class-I complex.Acquired homozygous loss of B2M that caused lack of cell-surface HLA Class I expression in the tumor and a matched patient-derived xenograft (PDX). Downregulation of B2M was also found in two additional PDXs established from ICI-resistant tumors.

Molecular Weight: 38.4 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: P01887

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process