CD80 Protein (CD80) (AA 35-242) (Fc Tag)

Catalog No. ABIN7273892

Recombinant CD80 protein expressed in HEK-293 Cells.

Quick Overview for CD80 Protein (CD80) (AA 35-242) (Fc Tag) (ABIN7273892)

Target: CD80

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 35-242

Purification tag / Conjugate: This CD80 protein is labelled with Fc Tag.

Purpose: Human B7-1/CD80 Protein

Sequence: Val35-Asn242

Characteristics: Recombinant Human B7-1/CD80 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val35-Asn242.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human B7-1, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, His Tag with the EC50 of 66ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD80

Alternative Name: B7-1

Background: Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.

Molecular Weight: 50.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

UniProt: P33681-1

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints