CD80 Protein (CD80) (AA 38-246) (His tag)
Quick Overview for CD80 Protein (CD80) (AA 38-246) (His tag) (ABIN7273895)
Target
See all CD80 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 38-246
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Purification tag / Conjugate
- This CD80 protein is labelled with His tag.
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Purpose
- Mouse B7-1/CD80 Protein
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Sequence
- Val38-Asn246
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Characteristics
- Recombinant Mouse B7-1/CD80 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val38-Asn246.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse B7-1 is greater than 95% as determined by SEC-HPLC.
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CD80 (CD80) (AA 35-242) (Active) protein (His-Avi Tag)
CD80 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD80 (CD80) (AA 35-242) (Active) protein (Biotin,His-Avi Tag)CD80 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD80 (CD80) (AA 35-242) protein (His tag,mFc Tag)CD80 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
CD80 (CD80) (AA 35-242) protein (His tag)HPLC verified CD80 Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
CD80 (CD80) (AA 137-230) protein (His-DYKDDDDK-Strep II Tag)CD80 Origin: Human Host: HEK-293T Cells Recombinant WB, ELISA, SDS, PI
CD80 (CD80) (AA 35-242) protein (His-DYKDDDDK-Strep II Tag)CD80 Origin: Human Host: HEK-293T Cells Recombinant WB, ELISA, SDS, PI
CD80 (CD80) (AA 38-245) (Active) protein (Fc Tag)CD80 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD80 (CD80) (AA 35-242) (Active) protein (Fc Tag,AVI tag,Biotin)CD80 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
CD80 (CD80) protein (His tag)CD80 Origin: Rabbit Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
CD80 (CD80) protein (Fc Tag)CD80 Origin: Cynomolgus Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD80
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Alternative Name
- B7-1
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Background
- Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
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Molecular Weight
- 24.6 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
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UniProt
- Q00609
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Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
Target
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