CD86 Protein (CD86) (AA 25-239) (His tag)

Catalog No. ABIN7273896

This Recombinant CD86 protein is produced in HEK-293 Cells.

Quick Overview for CD86 Protein (CD86) (AA 25-239) (His tag) (ABIN7273896)

Target: CD86

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-239

Purification tag / Conjugate: This CD86 protein is labelled with His tag.

Purpose: Cynomolgus B7-2/CD86 Protein

Sequence: Tyr25-His239

Characteristics: Recombinant Cynomolgus B7-2/CD86 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Tyr25-His239.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus B7-2, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 0.102 uM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA (QC Test).,Cynomolgus B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus B7-2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD86

Alternative Name: B7-2

Background: B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.

Molecular Weight: 26.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5TTJ8

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation