BAFF Protein (AA 141-285, Trimer) (His-DYKDDDDK Tag)

Catalog No. ABIN7273911

This Recombinant BAFF protein is produced in HEK-293 Cells.

Quick Overview for BAFF Protein (AA 141-285, Trimer) (His-DYKDDDDK Tag) (ABIN7273911)

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 141-285, Trimer

Purification tag / Conjugate: This BAFF protein is labelled with His-DYKDDDDK Tag.

Purpose: Human BAFF/TNFSF13B/CD257 Trimer Protein

Sequence: Thr141-Leu285

Characteristics: Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Thr141-Leu285 trimer design.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human BAFF Trimer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 6.59 nM as determined in SPR assay (Biacore T200).,Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA (QC Test).,Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.,Immobilized Human BAFF Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BAFF Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.,Human BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human TACI, hFc Tag on ProA-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 1.53 nM as determined in BLI assay (Gator® Prime).,Loaded Biotinylated Human BAFFR, His-Avi Tag on Streptavidin-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 0.20 nM as determined in BLI assay (Gator® Prime).,The purity of Human BAFF Trimer is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Alternative Name: BAFF

Background: B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

Molecular Weight: 52.4 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.

UniProt: Q9Y275

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response