TNFRSF13C Protein (AA 7-71) (Biotin,His-Avi Tag)

Catalog No. ABIN7273915

This Recombinant TNFRSF13C protein is expressed in HEK-293 Cells.

Quick Overview for TNFRSF13C Protein (AA 7-71) (Biotin,His-Avi Tag) (ABIN7273915)

Target: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 7-71

Purification tag / Conjugate: This TNFRSF13C protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human BAFFR/TNFRSF13C Protein

Sequence: Ser7-Ala71

Characteristics: Recombinant Biotinylated Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser7-Ala71.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human BAFF (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 0.42ug/ml determined by ELISA (QC Test).,Immobilized Anti-BAFFR Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 5.5ng/ml determined by ELISA.,Biotinylated Human BAFFR on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,Loaded Biotinylated Human BAFFR, His-Avi Tag on Streptavidin-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 0.20 nM as determined in BLI assay (Gator® Prime).,Loaded Biotinylated Human BAFFR, His-Avi Tag on Anti-His-Biosensor can bind Anti-BAFFR Ab., hFc Tag with an affinity constant of 18.5 pM as determined in BLI assay (Gator® Prime).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF13C (Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))

Alternative Name: BAFFR

Background: BAFFR is an atypical representative of the TNF-receptor super-family. BAFFR [BAFFR = B cell activating f actor of the TNF-f amily receptor, a.k.a. BR3, Bcmd] expression starts when the immature B cells develop to transitional B cells, which then receive BAFFR-dependent pro-survival signals to rescue them from premature cell death. At protein level, the BAFFR is expressed on the surface of all human peripheral B cell subsets except for plasma cells and for centroblasts located in the dark zone of germinal centers. Its expression is upregulated after the expression of functional B cell antigen receptors (BCR) in response to tonic BCR signaling, which enhances BAFFR expression by immature and transitional B cells

Molecular Weight: 9.5 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.

UniProt: Q96RJ3

Pathways: NF-kappaB Signaling