BAFF Protein (Trimer) (His-Avi Tag,Biotin)
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- Target See all BAFF (TNFSF13B) Proteins
- BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
- Protein Type
- Recombinant
- Protein Characteristics
- Trimer
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This BAFF protein is labelled with His-Avi Tag,Biotin.
- Sequence
- Thr141-Leu285
- Purity
- > 95% as determined by Tris-Bis PAGE,> 94% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human BAFFR, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human BAFF (Trimer) , His Tag with the EC50 of 6.9ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product TNFSF13B Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
- Alternative Name
- BAFF (TNFSF13B Products)
- Background
- BAFF, BLyS, CD257, TNFSF13B, TNFSF20, DTL, TALL1, TALL-1delta BAFF, TALL1Delta4 BAFF, THANK, ZTNF4, TALL-1,B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
- Molecular Weight
- 54.2 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9Y275
- Pathways
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response
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