CD137 Protein (AA 24-186) (Biotin,His-Avi Tag)

Catalog No. ABIN7273923

This Recombinant CD137 protein is produced in HEK-293 Cells.

Quick Overview for CD137 Protein (AA 24-186) (Biotin,His-Avi Tag) (ABIN7273923)

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-186

Purification tag / Conjugate: This CD137 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human 4-1BB/TNFRSF9 Protein

Sequence: Leu24-Gln186

Characteristics: Recombinant Biotinylated Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu24-Gln186.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test).,Biotinylated Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Alternative Name: 4-1BB

Background: 4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.

Molecular Weight: 20.2 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

UniProt: Q07011

Pathways: Cancer Immune Checkpoints