ICOSLG Protein (AA 19-258) (Biotin,His-Avi Tag)
Quick Overview for ICOSLG Protein (AA 19-258) (Biotin,His-Avi Tag) (ABIN7273968)
Target
See all ICOSLG ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 19-258
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Purification tag / Conjugate
- This ICOSLG protein is labelled with Biotin,His-Avi Tag.
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Purpose
- Biotinylated Human B7-H2/ICOSLG Protein
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Sequence
- Asp19-Ser258
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Characteristics
- Recombinant Biotinylated Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp19-Ser258.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human ICOS (C136S,C137S), His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 25.1ng/ml determined by ELISA.,Biotinylated Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human B7-H2 is greater than 90% as determined by SEC-HPLC.
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Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 19-258) (Active) protein (His-Avi Tag)
ICOSLG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 19-258) (Active) protein (hFc Tag)ICOSLG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 19-256) (Active) protein (His tag)ICOSLG Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 47-279) (Active) protein (His tag)ICOSLG Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 19-256) protein (His tag,mFc Tag)ICOSLG Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 18-135) protein (His-DYKDDDDK-Strep II Tag)ICOSLG Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 18-256) protein (His-DYKDDDDK-Strep II Tag)ICOSLG Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) protein (His tag)ICOSLG Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 20-302) protein (GST tag)ICOSLG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) (AA 19-258) (Active) protein (His tag)ICOSLG Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. WB, SDS, ELISA, Imm, AcA Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))
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Alternative Name
- B7-H2
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Background
- B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion, induces also B-cell proliferation and differentiation into plasma cells.
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Molecular Weight
- 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
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UniProt
- O75144
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Pathways
- Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
Target
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