B7-H6 Protein (AA 25-262) (Biotin,His-Avi Tag)

Catalog No. ABIN7273970

Recombinant B7-H6 protein expressed in HEK-293 Cells.

Quick Overview for B7-H6 Protein (AA 25-262) (Biotin,His-Avi Tag) (ABIN7273970)

Target: B7-H6 (NCR3LG1) (Natural Killer Cell Cytotoxicity Receptor 3 Ligand 1 (NCR3LG1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-262

Purification tag / Conjugate: This B7-H6 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human B7-H6/NCR3LG1 Protein

Sequence: Asp25-Ser262

Characteristics: Recombinant Biotinylated Human B7-H6/NCR3LG1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp25-Ser262.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human B7-H6, His-Avi Tag with an affinity constant of 0.227 uM as determined in SPR assay (Biacore T200).,Biotinylated Human B7-H6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human NKp30, hFc Tag on ProA-Biosensor can bind Biotinylated Human B7-H6, His-Avi Tag with an affinity constant of 0.17 μM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated Human B7-H6 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: B7-H6 (NCR3LG1) (Natural Killer Cell Cytotoxicity Receptor 3 Ligand 1 (NCR3LG1))

Alternative Name: B7-H6

Background: B7-H6 is a glycosylated member of the B7 family of immune co-stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.

Molecular Weight: 29.6 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result.

UniProt: Q68D85