ICOSLG Protein (AA 47-279) (His tag)

Catalog No. ABIN7273971

This Recombinant ICOSLG protein is produced in HEK-293 Cells.

Quick Overview for ICOSLG Protein (AA 47-279) (His tag) (ABIN7273971)

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 47-279

Purification tag / Conjugate: This ICOSLG protein is labelled with His tag.

Purpose: Mouse B7-H2/ICOSLG Protein

Sequence: Glu47-Lys279

Characteristics: Recombinant Mouse B7-H2/ICOSLG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu47-Lys279.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).,Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 36.2ng/ml determined by ELISA.,Mouse B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse B7-H2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Alternative Name: B7-H2

Background: B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion, induces also B-cell proliferation and differentiation into plasma cells.

Molecular Weight: 27.4 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Bis-Tris PAGE result.

UniProt: Q9JHJ8

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation