BMP2 Protein (AA 283-396)

Catalog No. ABIN7273975

This Recombinant BMP2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for BMP2 Protein (AA 283-396) (ABIN7273975)

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Dog, Rat, Mouse, Rhesus Monkey

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 283-396

Purpose: Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein

Sequence: Gln283-Arg396 (Human/Rhesus macaque) / Gln279-Arg393 (Mouse/Rat) / Gln282-Arg395 (Canine)

Characteristics: Recombinant Human/Rhesus macaque/Mouse/Rat/Canine BMP2 Protein is expressed from E.coli without tag.It contains Gln283-Arg396 (Human/Rhesus macaque) / Gln279-Arg393 (Mouse/Rat) / Gln282-Arg395 (Canine).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.5 EU per μg by the LAL method.

Biological Activity Comment: Human/Rhesus macaque/Mouse/Rat/Canine BMP2 on Bis-Tris PAGE under Non reduced (N) condition and reduced condition. The purity is greater than 95%.,Measured by its ability to induce Alkaline Phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is 40-200 ng/mL (QC Test).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 100 mM Acetic Acid ( pH 2.8). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 100 mM Acetic Acid ( pH 2.8). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Alternative Name: BMP2

Background: Bone morphogenetic protein 2 (BMP2), a member of the transforming growth factor-β (TGF-β) super-family, is one of the main chondrogenic growth factors involved in cartilage regeneration. BMP2 is known to induce chondrogenic differentiation in various types of stem cells in vitro. However, BMP2 also induces osteogenic differentiation and endochondral ossification in mesenchymal stem cells (MSCs).

Molecular Weight: 12.90 kDa same as Bis-Tris PAGE result.

UniProt: P12643

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation