Albumin Protein (ALB) (AA 25-608) (His tag)

Catalog No. ABIN7273985

Recombinant Albumin protein expressed in HEK-293 Cells.

Quick Overview for Albumin Protein (ALB) (AA 25-608) (His tag) (ABIN7273985)

Target: Albumin (ALB)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-608

Purification tag / Conjugate: This Albumin protein is labelled with His tag.

Purpose: Mouse Serum Albumin Protein

Sequence: Glu25-Ala608

Characteristics: Recombinant Mouse Serum Albumin Protein is expressed from HEK293 with His tag at the C-Terminus., It contains Glu25-Ala608.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Mouse Serum Albumin, His Tag immobilized on CM5 Chip can bind Mouse FcRn, His Tag with an affinity constant of 20.82 nM as determined in SPR assay (Biacore T200).,Mouse Serum Albumin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse Serum Albumin is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Albumin (ALB)

Alternative Name: Serum Albumin

Background: Albumins are multifunctional proteins present in the blood serum of animals. They can bind and transport a wide variety of ligands which they accommodate due to their conformational flexibility. Serum albumins are highly conserved both in amino acid sequence and three-dimensional structure. Several mammalian and avian serum albumins (SAs) are also allergens. Sensitization to one of the SAs coupled with the high degree of conservation between SAs may result in cross-reactive antibodies in allergic individuals.

Molecular Weight: 70.19 kDa same as Bis-Tris PAGE result.

UniProt: P07724

Pathways: Lipid Metabolism