CA9 Protein (AA 38-414) (His-Avi Tag)

Catalog No. ABIN7274014

This Recombinant CA9 protein is expressed in HEK-293 Cells.

Quick Overview for CA9 Protein (AA 38-414) (His-Avi Tag) (ABIN7274014)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 38-414

Purification tag / Conjugate: This CA9 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human CA9/Carbonic Anhydrase IX Protein

Sequence: Gln38-Asp414

Characteristics: Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, It contains Gln38-Asp414.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CA9, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 7.8 ng/ml determined by ELISA.,Human CA9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its esterase activity. The specific activity is >20 pmol/min/μg.,The purity of Human CA9 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: CA9

Background: CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.

Molecular Weight: 43.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

UniProt: Q16790