CA9 Protein (AA 38-414) (His-Avi Tag)
Quick Overview for CA9 Protein (AA 38-414) (His-Avi Tag) (ABIN7274014)
Target
See all CA9 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 38-414
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Purification tag / Conjugate
- This CA9 protein is labelled with His-Avi Tag.
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Purpose
- Human CA9/Carbonic Anhydrase IX Protein
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Sequence
- Gln38-Asp414
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Characteristics
- Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln38-Asp414.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human CA9, His Tag at 0.9μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA. See testing image for detail.
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Carbonic Anhydrase IX (CA9) (AA 38-402) protein (His tag)
CA9 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carbonic Anhydrase IX (CA9) (AA 32-390) protein (His tag)CA9 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carbonic Anhydrase IX (CA9) (AA 1-459) protein (GST tag)CA9 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase IX (CA9) (AA 38-414) protein (His tag)CA9 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Carbonic Anhydrase IX (CA9) (AA 38-398) protein (His tag)CA9 Origin: Cynomolgus Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Carbonic Anhydrase IX (CA9) (AA 38-153) protein (His-IF2DI Tag)CA9 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Carbonic Anhydrase IX (CA9) (Active) protein (His tag)CA9 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Carbonic Anhydrase IX (CA9) protein (His tag,Biotin)CA9 Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Carbonic Anhydrase IX (CA9) (AA 64-399), (partial) protein (GST tag)CA9 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % SDS, ELISA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in 50 mM Tris, 150 mM NaCl ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CA9 (Carbonic Anhydrase IX (CA9))
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Alternative Name
- CA9
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Background
- CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.
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Molecular Weight
- 43.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
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UniProt
- Q16790
Target
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