CGRP Protein (AA 26-119) (hFc Tag)

Catalog No. ABIN7274020

This Recombinant CGRP protein is expressed in HEK-293 Cells.

Quick Overview for CGRP Protein (AA 26-119) (hFc Tag) (ABIN7274020)

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-119

Purification tag / Conjugate: This CGRP protein is labelled with hFc Tag.

Purpose: Mouse CALCA/CGRP Protein

Sequence: Val26-Phe119

Characteristics: Recombinant Mouse CALCA/CGRP Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Val26-Phe119.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse CALCA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse CALCA is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Alternative Name: CALCA

Background: Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.

Molecular Weight: 36.9 kDa. Due to glycosylation, the protein migrates to 42-46 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_001029126

Pathways: Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour