EpCAM Protein (AA 24-265) (His tag)

Catalog No. ABIN7274023

This Recombinant EpCAM protein is expressed in HEK-293 Cells.

Quick Overview for EpCAM Protein (AA 24-265) (His tag) (ABIN7274023)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rhesus Monkey

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 24-265

Purification tag / Conjugate: This EpCAM protein is labelled with His tag.

Purpose: Rhesus macaque EpCAM/TROP1 Protein

Sequence: Gln24-Lys265

Characteristics: Recombinant Rhesus macaque EpCAM/TROP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln24-Lys265.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Rhesus macaque EpCAM, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-EpCAM Antibody, hFc Tag with an affinity constant of 21.40 nM as determined in SPR assay (Biacore T200).,Immobilized Rhesus macaque EpCAM, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 0.38ug/ml determined by ELISA (QC Test).,Rhesus macaque EpCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM

Background: Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF-like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.

Molecular Weight: 28.5 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_001035118