CA12 Protein (AA 25-301) (His tag)

Catalog No. ABIN7274033

Recombinant CA12 protein expressed in HEK-293 Cells.

Quick Overview for CA12 Protein (AA 25-301) (His tag) (ABIN7274033)

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-301

Purification tag / Conjugate: This CA12 protein is labelled with His tag.

Purpose: Human CA12/Carbonic anhydrase XII Protein

Sequence: Ala25-Ser301

Characteristics: Recombinant Human CA12/Carbonic anhydrase XII Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Ser301.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Alternative Name: carbonic anhydrase XII

Background: Carbonic anhydrases (CAs) are a family of enzymes involved in the pH regulation of metabolically active cells/tissues. Carbonic anhydrase XII (CA XII) is a key mediator of several signaling pathways that are involved in cancer development.

Molecular Weight: 32.22 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

UniProt: O43570-1