C3 Protein (AA 23-1663) (His tag)

Catalog No. ABIN7274036

Recombinant C3 protein expressed in HEK-293 Cells.

Quick Overview for C3 Protein (AA 23-1663) (His tag) (ABIN7274036)

Target: C3 (Complement Component 3 (C3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-1663

Purification tag / Conjugate: This C3 protein is labelled with His tag.

Purpose: Human Complement C3 Protein

Sequence: Ser23-Asn1663

Characteristics: Recombinant Human Complement C3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser23-Asn1663.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Complement C3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Complement C3 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement component 3

Background: Complement component 3 (C3), a pivotal molecule in the complement system, is an essential immune mediator in various diseases, including psoriasis. C3 deficiency promoted imiquimod-induced skin cell apoptosis and supported greater proportions of IFN-γ T cells in the inflamed tissues.

Molecular Weight: 188.52 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 67-68 kDa, 110-115 kDa and 140-160 kDa based on Bis-Tris PAGE result.

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling