CCL5 Protein (AA 24-91) (Biotin,His-Avi Tag)

Catalog No. ABIN7274046

This Recombinant CCL5 protein is produced in Escherichia coli (E. coli).

Quick Overview for CCL5 Protein (AA 24-91) (Biotin,His-Avi Tag) (ABIN7274046)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 24-91

Purification tag / Conjugate: This CCL5 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human CCL5 Protein

Sequence: Ser24-Ser91

Characteristics: Recombinant Biotinylated Human CCL5 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Ser24-Ser91.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CCL5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).,Biotinylated Human CCL5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 4 mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 4 mM HCI. Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: CCL5

Background: The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers.

Molecular Weight: 10.89 kDa same as Bis-Tris PAGE result.

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration