CCL24 Protein (AA 27-119) (His tag)

Catalog No. ABIN7274047

This Recombinant CCL24 protein is expressed in HEK-293 Cells.

Quick Overview for CCL24 Protein (AA 27-119) (His tag) (ABIN7274047)

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-119

Purification tag / Conjugate: This CCL24 protein is labelled with His tag.

Purpose: Human CCL24 Protein

Sequence: Val27-Cys119

Characteristics: Recombinant Human CCL24 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Val27-Cys119.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CCL24, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CCL24 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA (QC Test).,Human CCL24 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CCL24 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, 100 mM L-arginine ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Alternative Name: CCL24

Background: C-C motif chemokine ligand 24 (CCL24) is a chemokine that regulates inflammatory and fibrotic activities through its receptor, C-C motif chemokine receptor (CCR3). CCL24 is a chemokine that regulates inflammation and fibrosis. It was found to be significantly expressed in patients with non-alcoholic steatohepatitis, in whom it regulates profibrotic processes in the liver.

Molecular Weight: 11.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.

UniProt: O00175

Pathways: Regulation of Actin Filament Polymerization