CD19 Protein (AA 21-291) (His tag)

Catalog No. ABIN7274055

This Recombinant CD19 protein is produced in HEK-293 Cells.

Quick Overview for CD19 Protein (AA 21-291) (His tag) (ABIN7274055)

Target: CD19 (CD19 Molecule (CD19))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rhesus Monkey, Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 21-291

Purification tag / Conjugate: This CD19 protein is labelled with His tag.

Purpose: Cynomolgus/Rhesus macaque CD19 Protein

Sequence: Gln21-Trp291

Characteristics: Recombinant Cynomolgus/Rhesus macaque CD19 Protein is expressed from HEK293 with His tag at the N-terminus, It contains Gln21-Trp291.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.46 uM as determined in SPR assay (Biacore T200).,Cynomolgus/Rhesus macaque CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-CD19 Antibody, hFc Tag on ProA-Biosensor can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.27 μM as determined in BLI assay (Gator® Prime).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background: CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca²⁺ stores.

Molecular Weight: 30.96 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5W8L9

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway