CD160 Protein (CD160) (AA 25-158) (hFc Tag)
Quick Overview for CD160 Protein (CD160) (AA 25-158) (hFc Tag) (ABIN7274073)
Target
See all CD160 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 25-158
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Purification tag / Conjugate
- This CD160 protein is labelled with hFc Tag.
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Purpose
- Human CD160 Protein
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Sequence
- Gly25-Leu158
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Characteristics
- Recombinant Human CD160 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly25-Leu158.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human HVEM, His Tag at 5ug/ml (100 ul/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 84.6 ng/ml determined by ELISA.,Human CD160 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD160 is greater than 95% as determined by SEC-HPLC.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD160
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Alternative Name
- CD160
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Background
- CD160 (also Natural killer cell receptor BY55) is a 27 - 30 kDa member of the Ig superfamily. In human, it is expressed principally on nonmyeloid hematopoietic cells. CD160 antigenis a receptor on immune cells capable to deliver stimulatory or inhibitory signals that regulate cell activation and differentiation. Exists as a GPI-anchored and as a transmembrane form, each likely initiating distinct signaling pathways via phosphoinositol 3-kinase in activated NK cells and via LCK and CD247/CD3 zeta chain in activated T cells.
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Molecular Weight
- 41.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
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UniProt
- O95971
Target
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