CD200 Protein (CD200) (AA 56-257) (His tag)
Quick Overview for CD200 Protein (CD200) (AA 56-257) (His tag) (ABIN7274096)
Target
See all CD200 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 56-257
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Purification tag / Conjugate
- This CD200 protein is labelled with His tag.
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Purpose
- Cynomolgus CD200/OX-2 Protein
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Sequence
- Gln56-Gly257
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Characteristics
- Recombinant Cynomolgus CD200/OX-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln56-Gly257.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Cynomolgus CD200 R1, His Tag immobilized on CM5 Chip can bind Cynomolgus CD200, His Tag with an affinity constant of 35.84 nM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus CD200, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD200 R1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).,Cynomolgus CD200 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus CD200 is greater than 95% as determined by SEC-HPLC.
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CD200 (CD200) (AA 31-232) (Active) protein (His tag)
CD200 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD200 (CD200) (AA 31-232) protein (His tag)CD200 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, AM, ADA
CD200 (CD200) (Active) protein (His tag)CD200 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
CD200 (CD200) (AA 31-232) protein (Fc Tag)CD200 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, AM, ADA
CD200 (CD200) (AA 34-124) protein (GST tag)CD200 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD200 (CD200) (AA 31-232) protein (hIgG-His-tag)CD200 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % SDS
CD200 (CD200) (AA 31-232) protein (Fc Tag,AVI tag,Biotin)CD200 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD200
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Alternative Name
- CD200
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Background
- CD200 and its receptors are highly expressed in the lung, on epithelial cells and leukocytes, and emerging evidence links dysregulation of the CD200 pathway with asthma. Moreover, pharmacological modulation of CD200 receptors was shown to improve clinical and inflammatory outcomes of preclinical asthma models.
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Molecular Weight
- 23.66 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
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UniProt
- A0A2K5TQS2
Target
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