CD27 Protein (AA 21-192) (Fc Tag)
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- Target See all CD27 Proteins
- CD27 (CD27 Molecule (CD27))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-192
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD27 protein is labelled with Fc Tag.
- Purpose
- Human CD27/TNFRSF7 Protein
- Sequence
- Thr21-Ile192
- Characteristics
- Recombinant Human CD27/TNFRSF7 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Thr21-Ile192.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD27L Trimer at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product CD27 Protein
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CD27 Molecule (CD27) (AA 21-192) protein (His tag)
CD27 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD27 Molecule (CD27) (AA 20-191) protein (mFc-His Tag)CD27 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD27 Molecule (CD27) (AA 20-191) protein (Fc Tag)CD27 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD27 Molecule (CD27) (AA 21-192) protein (His tag)CD27 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD27 Molecule (CD27) (AA 20-191) protein (Fc Tag)CD27 Origin: Rhesus Monkey, Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD27 Molecule (CD27) (AA 24-182) protein (mFc Tag)CD27 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD27 Molecule (CD27) (AA 1-260) protein (GST tag)CD27 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD27 Molecule (CD27) (AA 21-192) (Active) protein (Fc Tag)CD27 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD27 Molecule (CD27) (AA 21-182) (Active) protein (Fc Tag)CD27 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD27 Molecule (CD27) protein (His tag,Fc Tag)CD27 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD27 (CD27 Molecule (CD27))
- Alternative Name
- CD27 (CD27 Products)
- Background
- CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co-stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
- Molecular Weight
- 46.2 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result.
- UniProt
- P26842
- Pathways
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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