CD27 Protein (AA 21-192) (His tag)

Catalog No. ABIN7274097

Product Details

Target: CD27 (CD27 Molecule (CD27))

Protein Type: Recombinant

Protein Characteristics: AA 21-192

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This CD27 protein is labelled with His tag.

Purpose: Cynomolgus CD27/TNFRSF7 Protein

Sequence: Thr21-Ile192

Characteristics: Recombinant Cynomolgus CD27/TNFRSF7 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr21-Ile192.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus CD27, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 68.8ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27 (CD27 Products)

Synonyms: TNFRSF7 Protein, S152 Protein, Tnfrsf7 Protein, Tp55 Protein, S152. LPFS2 Protein, T14 Protein, CD27 molecule Protein, CD27 antigen Protein, CD27 Protein, Cd27 Protein

Background: CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co-stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.

Molecular Weight: 20.41 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005569963

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints