CD24 Protein (AA 27-59) (Llama Fc Tag)

Catalog No. ABIN7274119

This Recombinant CD24 protein is expressed in HEK-293 Cells.

Quick Overview for CD24 Protein (AA 27-59) (Llama Fc Tag) (ABIN7274119)

Target: CD24 (CD24 Molecule (CD24))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-59

Purification tag / Conjugate: This CD24 protein is labelled with Llama Fc Tag.

Purpose: Human CD24 Protein

Sequence: Ser27-Gly59

Characteristics: Recombinant Human CD24 Protein is expressed from HEK293 with Llama Fc tag at the C-Terminus.It contains Ser27-Gly59.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD24, Llama Fc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD24 Antibody, hFc Tag with the EC50 of 0.18ug/ml determined by ELISA (QC Test).,Human CD24 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD24 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD24 (CD24 Molecule (CD24))

Alternative Name: CD24

Background: CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.

Molecular Weight: 30.4 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.

UniProt: P25063

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation