CD3D & CD3E (AA 22-117) (Active) protein (hFc Tag)

Catalog No. ABIN7274130

This Recombinant CD3D & CD3E protein is produced in HEK-293 Cells.

Quick Overview for CD3D & CD3E (AA 22-117) (Active) protein (hFc Tag) (ABIN7274130)

Target: CD3D & CD3E

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-117

Purification tag / Conjugate: hFc Tag

Purpose: Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein

Sequence: Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D)

Specificity: Uni-Prot: Q95LI5.2 (CD3E), Q95LI8 (CD3D)

Characteristics: Recombinant Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus of CD3E and CD3D., It contains Gln22-Asp117 (CD3E) and Phe22-Ala105 (CD3D).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus CD3E&CD3D, hFc Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Antibody, hFc Tag with EC50 of 14.2ng/ml determined by ELISA.,Cynomolgus CD3E&CD3D on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Cynomolgus CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD3D & CD3E

Alternative Name: CD3E&CD3D

Background: T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Molecular Weight: 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 58-62 kDa (CD3E) and 44-46 kDa (CD3D) based on Bis-Tris PAGE result.