CD38 Protein (AA 43-300) (His tag)
Quick Overview for CD38 Protein (AA 43-300) (His tag) (ABIN7274142)
Target
See all CD38 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 43-300
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Purification tag / Conjugate
- This CD38 protein is labelled with His tag.
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Purpose
- Human CD38 Protein
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Sequence
- Val43-Ile300
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Characteristics
- Recombinant Human CD38 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val43-Ile300.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human CD38, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 28ng/ml determined by ELISA. The affinity constant of 0.23μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD38
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Background
- CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase, is a transmembrane glycoprotein found on the surface of some immune cells including plasma cells, activated or immature T and B cells, monocytes, and natural killer cells. CD38 participates in cell adhesion, signal transduction and calcium signaling.
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Molecular Weight
- 30.7 kDa. Due to glycosylation, the protein migrates to 45-58 kDa based on Tris-Bis PAGE result.
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UniProt
- P28907-1
Target
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