CD3 epsilon Protein (CD3E) (AA 23-48) (hFc-Avi Tag)

Catalog No. ABIN7274153

Recombinant CD3 epsilon protein expressed in HEK-293 Cells.

Quick Overview for CD3 epsilon Protein (CD3E) (AA 23-48) (hFc-Avi Tag) (ABIN7274153)

Target: CD3 epsilon (CD3E)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-48

Purification tag / Conjugate: This CD3 epsilon protein is labelled with hFc-Avi Tag.

Purpose: Non-biotinylated Human CD3E/CD3 epsilon 1-27 Protein

Sequence: Asp23-Thr48

Characteristics: Recombinant Human CD3E/CD3 epsilon 1-27 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Asp23-Thr48.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD3E 1-27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA.,Human CD3E 1-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD3E 1-27 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Molecular Weight: 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway