TNFRSF8 Protein (AA 19-379) (His-Avi Tag)

Catalog No. ABIN7274157

This Recombinant TNFRSF8 protein is produced in HEK-293 Cells.

Quick Overview for TNFRSF8 Protein (AA 19-379) (His-Avi Tag) (ABIN7274157)

Target: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-379

Purification tag / Conjugate: This TNFRSF8 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human CD30/TNFRSF8 Protein

Sequence: Phe19-Lys379

Characteristics: Recombinant Human CD30/TNFRSF8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe19-Lys379.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD30, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 21.4ng/ml determined by ELISA.,Immobilized Human CD30, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD30 Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA.,Human CD30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD30 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Alternative Name: CD30

Background: The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs).

Molecular Weight: 41.3 kDa. Due to glycosylation, the protein migrates to 68-95 kDa based on Bis-Tris PAGE result.

UniProt: P28908