CD33 Protein (CD33) (AA 18-259) (Biotin,His-Avi Tag)
Quick Overview for CD33 Protein (CD33) (AA 18-259) (Biotin,His-Avi Tag) (ABIN7274160)
Target
See all CD33 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 18-259
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Purification tag / Conjugate
- This CD33 protein is labelled with Biotin,His-Avi Tag.
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Purpose
- Biotinylated Human Siglec-3/CD33 Protein
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Sequence
- Asp18-His259
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Characteristics
- Recombinant Biotinylated Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp18-His259.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Biotinylated Human Siglec-3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.,Biotinylated Human Siglec-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human Siglec-3 is greater than 95% as determined by SEC-HPLC.
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CD33 (CD33) (AA 18-259) (Active) protein (His-Avi Tag)
CD33 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD33 (CD33) (AA 18-259) (Active) protein (hFc Tag)CD33 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD33 (CD33) (AA 17-248) (Active) protein (His tag)CD33 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD33 (CD33) (AA 18-259) protein (His tag,Fc Tag)CD33 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, AM, ADA
CD33 (CD33) protein (His tag)CD33 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
CD33 (CD33) (AA 1-364) protein (GST tag)CD33 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
CD33 (CD33) (AA 18-259) (Active) protein (His-Fc tag)CD33 Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. ELISA, WB, SDS, AcA, Imm Active
CD33 (CD33) (AA 140-259) protein (Fc Tag)CD33 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, AM, ADA
CD33 (CD33) (AA 18-240) protein (Fc Tag)CD33 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, AM, ADA
CD33 (CD33) (AA 18-259) protein (His tag)CD33 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as 0.22μm filtered solution in 50 mM MES, 500 mM NaCl, 200 mM L-arginine ( pH 7.4).
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Storage
- -80 °C
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Storage Comment
- Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD33
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Alternative Name
- Siglec-3
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Background
- CD33 (siglec-3), a well-known target in leukemia therapy, is an inhibitory sialoadhesin expressed in human leukocytes of the myeloid lineage and some lymphoid subsets, including NK cells. CD33 is the smallest siglec member. It preferentially binds to α2-6- and α2-3-sialylated glycans and strongly binds to sialylated ligands on leukemic cell lines.
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Molecular Weight
- 29.6 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.
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UniProt
- P20138
Target
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