CD43 Protein (AA 20-253) (His tag)
Quick Overview for CD43 Protein (AA 20-253) (His tag) (ABIN7274178)
Target
See all CD43 (SPN) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 20-253
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Purification tag / Conjugate
- This CD43 protein is labelled with His tag.
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Purpose
- Human CD43 Protein
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Sequence
- Ser20-Arg253
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Characteristics
- Recombinant Human CD43 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser20-Arg253.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human CD43 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD43 (SPN) (Sialophorin (SPN))
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Alternative Name
- CD43
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Background
- CD43 (leukosialin) is a large sialoglycoprotein abundantly expressed on the surface of most cells from the hematopoietic lineage. CD43 is directly involved in the contact between cells participating in a series of events such as signaling, adherence and host parasite interactions. The induction of acute myocarditis involves the engagement of CD43 cytoplasmic tripeptide sequence KRR to ezrin-radixin-moiesin cytoskeletal proteins.
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Molecular Weight
- 24.5 kDa. Due to glycosylation, the protein migrates to 50-80 kDa based on Bis-Tris PAGE result.
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UniProt
- P16150
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Pathways
- Regulation of Leukocyte Mediated Immunity
Target
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