CD46 Protein (CD46) (AA 35-343) (His tag)
-
- Target See all CD46 Proteins
- CD46
- Protein Type
- Recombinant
- Protein Characteristics
- AA 35-343
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD46 protein is labelled with His tag.
- Purpose
- Human CD46 Protein
- Sequence
- Cys35-Asp343
- Characteristics
- Recombinant Human CD46 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys35-Asp343.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD46, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD46 Antibody, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA. See testing image for detail.
-
CD46 (CD46) (AA 35-329) protein (His tag)
CD46 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD46 (CD46) (AA 45-344) protein (His tag)CD46 Origin: Dog Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD46 (CD46) (AA 35-328) protein (His tag)CD46 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD46 (CD46) (AA 35-313) protein (His tag)CD46 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
CD46 (CD46) (AA 35-313) protein (His tag)CD46 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
CD46 (CD46) (AA 1-384) protein (GST tag)CD46 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- CD46
- Alternative Name
- CD46 (CD46 Products)
- Background
- CD46 was discovered in 1986 during a search for novel C3b-binding proteins. CD46 is expressed ubiquitously and functions as a co-factor in the factor I-mediated proteolytic cleavage of C3b and C4b. Its vital role in preventing complement deposition on host tissue is underpinned by the fact that deficiency of CD46 is a predisposing factor for numerous disease conditions arising from complement-mediated 'self-attack'.
- Molecular Weight
- 35.4 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
- UniProt
- P15529-1
- Pathways
- Regulation of Actin Filament Polymerization
-
