CD46 Protein (CD46) (AA 35-329) (His tag)

Catalog No. ABIN7274175

Product Details

Target: CD46

Protein Type: Recombinant

Protein Characteristics: AA 35-329

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This CD46 protein is labelled with His tag.

Purpose: Cynomolgus CD46 Protein

Sequence: Cys35-Asp329

Characteristics: Recombinant Cynomolgus CD46 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys35-Asp329.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus CD46, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD46 Antibody, hFc Tag with the EC50 of 0.67μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD46

Alternative Name: CD46 (CD46 Products)

Synonyms: AHUS2 Protein, MCP Protein, MIC10 Protein, TLX Protein, TRA2.10 Protein, Mcp Protein, CD46 Protein, CD46 molecule Protein, CD46 antigen, complement regulatory protein Protein, CD46 molecule, complement regulatory protein Protein, membrane cofactor protein-like Protein, CD46 Protein, Cd46 Protein, LOC100341359 Protein, LOC103789310 Protein

Background: CD46 was discovered in 1986 during a search for novel C3b-binding proteins. CD46 is expressed ubiquitously and functions as a co-factor in the factor I-mediated proteolytic cleavage of C3b and C4b. Its vital role in preventing complement deposition on host tissue is underpinned by the fact that deficiency of CD46 is a predisposing factor for numerous disease conditions arising from complement-mediated 'self-attack'.

Molecular Weight: 34.05 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5WCS2

Pathways: Regulation of Actin Filament Polymerization