CD4 Protein (CD4) (AA 26-390) (Biotin,His-Avi Tag)

Catalog No. ABIN7274185

This Recombinant CD4 protein is expressed in HEK-293 Cells.

Quick Overview for CD4 Protein (CD4) (AA 26-390) (Biotin,His-Avi Tag) (ABIN7274185)

Target: CD4

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-390

Purification tag / Conjugate: This CD4 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human CD4/LEU3 Protein

Sequence: Lys26-Trp390

Characteristics: Recombinant Biotinylated Human CD4/LEU3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys26-Trp390.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CD4 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 8.4ng/ml determined by ELISA.,Biotinylated Human CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human CD4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: CD4

Alternative Name: CD4

Background: CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.

Molecular Weight: 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events