CD43 Protein (AA 20-248) (His tag)
Quick Overview for CD43 Protein (AA 20-248) (His tag) (ABIN7274189)
Target
See all CD43 (SPN) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 20-248
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Purification tag / Conjugate
- This CD43 protein is labelled with His tag.
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Purpose
- Mouse CD43 Protein
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Sequence
- Asp20-Gly248
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Characteristics
- Recombinant Mouse CD43 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp20-Gly248.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse CD43 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse CD43 is greater than 95% as determined by SEC-HPLC.
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Sialophorin (SPN) (AA 20-253) protein (Fc Tag)
SPN Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Sialophorin (SPN) (AA 20-253) (Active) protein (His tag)SPN Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE Active
Sialophorin (SPN) (AA 1-400) protein (GST tag)SPN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sialophorin (SPN) protein (Fc Tag)SPN Origin: Mouse Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE
Sialophorin (SPN) (AA 27-253) protein (His tag)SPN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Sialophorin (SPN) (AA 20-253) protein (His tag)SPN Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD43 (SPN) (Sialophorin (SPN))
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Alternative Name
- CD43
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Background
- CD43 is a large transmembrane protein involved in T cell activation. Previous studies of CD43-/- mice in viral models have demonstrated a role for CD43 in Th1/Th2 skewing, activation of Foxp3 Treg, and T cell apoptosis. CD43 (leukosialin) is a large sialoglycoprotein abundantly expressed on the surface of most cells from the hematopoietic lineage. CD43 is directly involved in the contact between cells participating in a series of events such as signaling, adherence and host parasite interactions.
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Molecular Weight
- 23.6 kDa. Due to glycosylation, the protein migrates to 80-120 kDa based on Bis-Tris PAGE result.
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UniProt
- P15702
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Pathways
- Regulation of Leukocyte Mediated Immunity
Target
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