CD5 Protein (CD5) (AA 25-375) (His-Avi Tag)
Quick Overview for CD5 Protein (CD5) (AA 25-375) (His-Avi Tag) (ABIN7274209)
Target
See all CD5 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 25-375
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Purification tag / Conjugate
- This CD5 protein is labelled with His-Avi Tag.
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Purpose
- Non-biotinylated Rhesus macaque CD5 Protein
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Sequence
- Arg25-Pro375
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Characteristics
- Recombinant Rhesus macaque CD5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg25-Pro375.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per ug by the LAL method.
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Biological Activity Comment
- Immobilized Rhesus macaque CD5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA.,Rhesus macaque CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Rhesus macaque CD5 is greater than 95% as determined by SEC-HPLC.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD5
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Alternative Name
- CD5
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Background
- CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion, expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
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Molecular Weight
- 41.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
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UniProt
- F6V5Q9
Target
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