CD6 Protein (CD6) (AA 18-402) (His tag)

Catalog No. ABIN7274212

This Recombinant CD6 protein is expressed in HEK-293 Cells.

Quick Overview for CD6 Protein (CD6) (AA 18-402) (His tag) (ABIN7274212)

Target: CD6

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 18-402

Purification tag / Conjugate: This CD6 protein is labelled with His tag.

Purpose: Human CD6 Protein

Sequence: His18-Leu402

Characteristics: Recombinant Human CD6 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His18-Leu402.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 uM as determined in SPR assay (Biacore T200).,Immobilized Human CD6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test).,Human CD6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD6 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD6

Alternative Name: CD6

Background: The cell surface glycoprotein CD6 is expressed on leukocytes and mediates T cell trafficking across endothelial cell barriers. CD6 is a lymphocyte surface co-receptor physically associated with the T-cell receptor (TCR)/CD3 complex at the center of the immunological synapse.

Molecular Weight: 42.14 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_006716