CD70 Protein (AA 39-193) (Fc Tag)
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- Target See all CD70 Proteins
- CD70 (CD70 Molecule (CD70))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 39-193
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD70 protein is labelled with Fc Tag.
- Purpose
- Human CD27 Ligand/CD70 Protein
- Sequence
- Gln39-Pro193
- Characteristics
- Recombinant Human CD27 Ligand/CD70 Protein is expressed from HEK293 with hFc at the N-Terminus.It contains Gln39-Pro193.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD27, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 37.9ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD70 (CD70 Molecule (CD70))
- Alternative Name
- CD27 Ligand (CD70 Products)
- Synonyms
- CD27LG Protein, Cd27l Protein, Tnfsf7 Protein, CD27L Protein, TNFSF7 Protein, CD70 antigen Protein, CD70 molecule Protein, Cd70 molecule Protein, Cd70 Protein, CD70 Protein
- Background
- CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7. CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
- Molecular Weight
- 42.7 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.
- Pathways
- Cancer Immune Checkpoints
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