CD79b Protein (AA 29-159) (His-Avi Tag)
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- Target See all CD79b (CD79B) Proteins
- CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-159
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD79b protein is labelled with His-Avi Tag.
- Purpose
- Human CD79B Protein
- Sequence
- Ala29-Asp159
- Characteristics
- Recombinant Human CD79B Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala29-Asp159.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD79B, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-CD79B Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))
- Alternative Name
- CD79B (CD79B Products)
- Synonyms
- B29 Protein, Ig-beta Protein, Igb Protein, Igbeta Protein, AGM6 Protein, IGB Protein, CD79B antigen Protein, CD79b molecule Protein, Cd79b Protein, CD79B Protein
- Background
- CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta-chain) is a 36-40 kDa member of the Ig-Superfamily. It is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
- Molecular Weight
- 18.1 kDa. Due to glycosylation, the protein migrates to 33-42 kDa based on Tris-Bis PAGE result.
- Pathways
- BCR Signaling
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