CD70 Protein (AA 45-195) (hFc Tag)

Catalog No. ABIN7274243

Recombinant CD70 protein expressed in HEK-293 Cells.

Quick Overview for CD70 Protein (AA 45-195) (hFc Tag) (ABIN7274243)

Target: CD70 (CD70 Molecule (CD70))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 45-195

Purification tag / Conjugate: This CD70 protein is labelled with hFc Tag.

Purpose: Mouse CD27 Ligand/CD70 Protein

Sequence: Ser45-Pro195

Characteristics: Recombinant Mouse CD27 Ligand/CD70 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Ser45-Pro195.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Mouse CD27, mFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA.,Mouse CD27 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse CD27 Ligand is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 6.2). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: CD70 (CD70 Molecule (CD70))

Alternative Name: CD27 Ligand

Background: CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.  CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

Molecular Weight: 43.41 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.

UniProt: O55237

Pathways: Cancer Immune Checkpoints