CD70 Protein (AA 45-195) (Fc Tag)

Catalog No. ABIN7274243

Product Details

Target: CD70 (CD70 Molecule (CD70))

Protein Type: Recombinant

Protein Characteristics: AA 45-195

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This CD70 protein is labelled with Fc Tag.

Purpose: Mouse CD27 Ligand/CD70 Protein

Sequence: Ser45-Pro195

Characteristics: Recombinant Mouse CD27 Ligand/CD70 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ser45-Pro195.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Mouse CD27, mFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 6.2). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD70 (CD70 Molecule (CD70))

Alternative Name: CD27 Ligand (CD70 Products)

Synonyms: CD27LG Protein, Cd27l Protein, Tnfsf7 Protein, CD27L Protein, TNFSF7 Protein, CD70 antigen Protein, CD70 molecule Protein, Cd70 molecule Protein, Cd70 Protein, CD70 Protein

Background: CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.  CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

Molecular Weight: 43.41 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.

UniProt: O55237

Pathways: Cancer Immune Checkpoints