FCGR2A Protein (AA 28-208) (His tag)

Catalog No. ABIN7274277

This Recombinant FCGR2A protein is produced in HEK-293 Cells.

Quick Overview for FCGR2A Protein (AA 28-208) (His tag) (ABIN7274277)

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 28-208

Purification tag / Conjugate: This FCGR2A protein is labelled with His tag.

Purpose: Cynomolgus Fc gamma RIIA/CD32a Protein

Sequence: Gln28-Pro208

Characteristics: Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln28-Pro208.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus Fc gamma RIIA, His Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 4.39 uM as determined in SPR assay (Biacore T200).,Cynomolgus Fc gamma RIIA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Cynomolgus Fc gamma RIIA, His Tag on Anti-His-Biosensor, can bind Rituximab with an affinity constant of 1.75 μM as determined in BLI assay (Gator).,The purity of Cynomolgus Fc gamma RIIA is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Alternative Name: Fc gamma RIIA

Background: The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains, these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.

Molecular Weight: 21.3 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

UniProt: Q8SPW4