CD300a Protein (CD300A) (AA 18-180) (His tag)

Catalog No. ABIN7274278

This Recombinant CD300a protein is expressed in HEK-293 Cells.

Quick Overview for CD300a Protein (CD300A) (AA 18-180) (His tag) (ABIN7274278)

Target: CD300a (CD300A)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 18-180

Purification tag / Conjugate: This CD300a protein is labelled with His tag.

Purpose: Human CD300A Protein

Sequence: Leu18-Pro180

Characteristics: Recombinant Human CD300A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu18-Pro180.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD300A, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.,Human CD300A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD300A is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: CD300a (CD300A)

Alternative Name: CD300A

Background: CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI), however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation.

Molecular Weight: 18.7 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result.

UniProt: Q9UGN4

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process