CD1a Protein (CD1a) (AA 19-300) (His tag)

Catalog No. ABIN7274279

Recombinant CD1a protein expressed in HEK-293 Cells.

Quick Overview for CD1a Protein (CD1a) (AA 19-300) (His tag) (ABIN7274279)

Target: CD1a

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-300

Purification tag / Conjugate: This CD1a protein is labelled with His tag.

Purpose: Human CD1A Protein

Sequence: Asp19-Val300

Characteristics: Recombinant Human CD1A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp19-Val300.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human CD1A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD1A is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD1a

Alternative Name: CD1A

Background: CD1 proteins are a family of major histocompatibility complex (MHC) class I-like antigen-presenting molecules that present lipids to T cells. The cytoplasmic tails (CTs) of all human CD1 isoforms, with the exception of CD1a, contain tyrosine-based sorting motifs, responsible for the internalization of proteins by the clathrin-mediated pathway. CD1a closer to MHC class I in its trafficking and potential antigen-loading compartments among CD1 isoforms.

Molecular Weight: 33.3 kDa. Due to glycosylation, the protein migrates to 48-53 kDa and 13-14 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_001754

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process