FCGR3A Protein (AA 17-208) (His-Avi Tag,Biotin)
Quick Overview for FCGR3A Protein (AA 17-208) (His-Avi Tag,Biotin) (ABIN7274282)
Target
See all FCGR3A ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 17-208
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Purification tag / Conjugate
- This FCGR3A protein is labelled with His-Avi Tag,Biotin.
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Purpose
- Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein
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Sequence
- Gly17-Gln208(F176)
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Characteristics
- Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly17-Gln208(F176).
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA. The affinity constant of 0.14μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-208) protein (His-Avi Tag)
FCGR3A Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-208) protein (His-Avi Tag,Biotin)FCGR3A Origin: Human Host: HEK-293 Cells Recombinant > 95 % by SEC-HPLC SDS, SEC-HPLC, SPR
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-254) protein (GST tag)FCGR3A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-208) (Active) protein (His tag)FCGR3A Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) protein (His tag)FCGR3A Origin: Cynomolgus Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-206) protein (His tag)FCGR3A Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (Active) protein (His tag)FCGR3A Origin: Human Host: HEK-293 Cells Recombinant > 97 % as determined by reducing SDS-PAGE. Active
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (Active) protein (His tag,AVI tag)FCGR3A Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (Active) protein (His tag)FCGR3A Origin: Rat Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (Active) protein (Biotin,His-Avi Tag)FCGR3A Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
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Alternative Name
- Fc gamma RIIIA
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Background
- Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
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Molecular Weight
- 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
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UniProt
- P08637-1
Target
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