FCGR2B Protein (AA 40-217) (His tag)

Catalog No. ABIN7274290

This Recombinant FCGR2B protein is expressed in HEK-293 Cells.

Quick Overview for FCGR2B Protein (AA 40-217) (His tag) (ABIN7274290)

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 40-217

Purification tag / Conjugate: This FCGR2B protein is labelled with His tag.

Purpose: Mouse Fc gamma RIIB/CD32b Protein

Sequence: Thr40-Arg217

Characteristics: Recombinant Mouse Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr40-Arg217.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIIB, His Tag with an affinity constant of 0.280 uM as determined in SPR assay (Biacore T200).,Mouse Fc gamma RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded OKT3 on ProA-Biosensor can bind Mouse Fc gamma RIIB,His Tag with an affinity constant of 1.99 μM as determined in BLI assay (Gator).,The purity of Mouse Fc gamma RIIB is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Alternative Name: Fc gamma RIIB

Background: The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains, these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.

Molecular Weight: 21.4 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_001070657

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling