CDCP1 Protein (AA 30-666) (His tag)

Catalog No. ABIN7274293

This Recombinant CDCP1 protein is expressed in HEK-293 Cells.

Quick Overview for CDCP1 Protein (AA 30-666) (His tag) (ABIN7274293)

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 30-666

Purification tag / Conjugate: This CDCP1 protein is labelled with His tag.

Purpose: Human CDCP1 Protein

Sequence: Phe30-Leu666

Characteristics: Recombinant Human CDCP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe30-Leu666.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human CDCP1, His Tag captured on CM5 Chip via anti-His antibody can bind Anti-CDCP1 Antibody, hFc Tag with an affinity constant of 0.18nM as determined in SPR assay (Biacore T200).,Immobilized Human CDCP1 at 2ug/ml (100ul/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).,Human CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CDCP1 is greater than 95% and the molecular weight of this protein is around 80-105 kDa as determined by SEC-MALS.,Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Human CDCP1, His Tag with an affinity constant of 17.00 nM as determined in BLI assay (Gator® Prime).,The purity of Human CDCP1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Alternative Name: CDCP1

Background: Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Molecular Weight: 72.8 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.

UniProt: Q9H5V8