CDCP1 Protein (His tag)

Catalog No. ABIN7274293

Product Details

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CDCP1 protein is labelled with His tag.

Sequence: Phe30-Leu666

Purity: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CDCP1 at 2μg/ml (100μl/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Storage: 4 °C,-80 °C

Storage Comment: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Alternative Name: CDCP1 (CDCP1 Products)

Background: CDCP1, CD318, SIMA135, TRASK, UNQ2486, PRO5773,Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Molecular Weight: 72.8 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.